The Living Brain Project Summary - The Test That Can Determine Health & Longevity

Article at a glance

  • Omega 3 Index, Omega-6: Omega-3 ratio, and AA:EPA ratio, are three health indicators that have been proven to indicate an individual’s disease risk, inflammation status, and overall mortality risk.
  • 56 human volunteers across all ages supplemented their daily routine with Brain Armor’s Omega-3+ supplement for 90 days to test how effective it was at raising these three health indicators.
  • After 90 days of supplementation: the average O3i increased from 5.59% to 8.8% (improved health outcome); the Omega-6: Omega-3 ratio dropped from 7.35:1 to 4.5:1 (improved health outcome); and the average AA: EPA ratio dropped from 21.8:1 to 7.76:1 (improved health outcome).
  • The results of this study indicate that supplementing daily with Brain Armor’s Omega-3+ for at least 90 days can improve indicators of inflammation, disease risk, and all cause mortality risk in several age populations. 


Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have significant health benefits, and now there is a health indicator influenced by these nutrients that can tell you even more about your overall health and disease risk than your own cholesterol levels. 

Evidence has indicated a direct link between a person’s Omega-3 Index (O3i) score and their longevity. An O3i is a simple blood test that reflects the percentage of Omega-3 fatty acids present in your red blood cell membranes. Specifically, studies show that an optimal O3i score is >8% while an index of <4% may be regarded as a risk factor for disease and mortality. 

In addition to the O3i, research has concluded that a person’s Omega-6: Omega-3 ratio can also indicate a lot about their health risk. The typical Western diet provides a ratio close to 16:1 Omega-6: Omega-3, but studies suggest that reducing that ratio to 2-3:1 will suppress inflammation and minimize the risk of chronic disease. Similarly, high arachidonic acid (AA): EPA ratio has also been linked to bodily inflammation and certain types of cancers, whereas lower ratios are more desirable for long term health and longevity. 

Can we improve our health outcomes and decrease the risk of chronic disease by ensuring an adequate intake of high-quality omega-3s? The Living Brain Project set out to determine that by monitoring the effects of daily dosing with Brain Armor’s Omega-3+ formula on the participant’s O3i, Omega-6: Omega-3 ratio, and AA: EPA ratio.  

Study Design and Results 

56 human volunteers (46 males, 10 females) aged 3 to 66 years old participated in this study. The participants resided throughout the United States and exhibited different health histories, daily food intake, and physical activity practices. Each participant took an initial Omega Quant O3i test to establish a baseline O3i, Omega6: Omega-3, and AA: EPA. The participants were then instructed to take a daily dose of Brain Armor’s Omega-3+ supplement that was compatible for their age and to remain consistent with all other lifestyle variables for 90 days. Each participant then followed up with a secondary Omega Quant O3i test to determine their test levels post supplementation. 

100% of the participants who supplemented with Brain Armor’s Omega-3+ supplement for 90 days demonstrated an increase in O3i levels, even those who were supplementing with a different source of Omega-3 before the start of the study. The participant’s average O3i went from 5.59% at baseline to 8.8% 90 days later. Similar results were seen with the Omega-6: Omega-3 ratio and AA: EPA ratio. The average Omega-6: Omega-3 ratio dropped from a 7.35% at baseline to 4.5%, and the AA: EPA ratio average dropped from 21.8:1 at baseline to a 7.76:1 after 90 days of supplementation.

This study demonstrated that a person could improve their O3i, Omega-6: Omega-3 ratio, AA: EPA ratio, and potentially their health risk by taking Brain Armor’s Omega-3+ supplement daily for 90 days. Since a number of participants were regularly taking an Omega-3 supplement derived from fish or krill oil prior to the start of the study, it can be concluded that the combination of ingredients found in Brain Armor Omega-3+ supplements may be more effective at raising O3i levels than obtaining them from a whole food diet or from another type of supplementation. Additionally, this study demonstrated that both the Omega 6: Omega 3 ratio, and the AA: EPA ratio, can be improved through diet, and results show that intake of Omega-3s of approximately 2.5g per day is sufficient to bring the AA: EPA ratio into the desired range for optimum wellness. 

Do you know your O3i number?

Stacy Cappadona MS, RD, CSCS received her BS in Exercise Science and MS in Exercise Physiology and Sports Nutrition from Florida State University. She has worked with athletes of all ages, active duty military personnel, and is now serving people globally through virtual coaching programs as the Founder of Stacy Rae Wellness